p38 MAPK Recombinant antibody
p38 MAPK Recombinant Antibody for WB, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, FC (Intra), IP, ELISA
Conjugate
Unconjugated
CloneNo.
230263H7
Cat no : 80821-3-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue |
Positive IP detected in | HEK-293 cells |
Positive FC (Intra) detected in | HeLa cells, Jurkat cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
80821-3-RR targets p38 MAPK in WB, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | p38 MAPK fusion protein Ag5115 相同性解析による交差性が予測される生物種 |
Full Name | mitogen-activated protein kinase 14 |
Calculated molecular weight | 360 aa, 41 kDa |
Observed molecular weight | 38-42 kDa |
GenBank accession number | BC031574 |
Gene symbol | p38 MAPK |
Gene ID (NCBI) | 1432 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cell self-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has 4 isoforms produced by alternative splicing.
Protocols
Product Specific Protocols | |
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WB protocol for p38 MAPK antibody 80821-3-RR | Download protocol |
IP protocol for p38 MAPK antibody 80821-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |