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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated p63 Polyclonal antibody

p63 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12143

Synonyms

AIS, B(p51A), B(p51B), CUSP, deltaNp63, EEC3, KET, LMS, NBP, OFC8, p40, p51, p53CP, p63, p73H, p73L, RHS, SHFM4, TP53CP, TP53L, TP63, TP73L, Tumor protein 63, tumor protein p63, Tumor protein p73 like

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated p63 Polyclonal antib (CL488-12143)

    IF Staining of A431 using CL488-12143

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 p63 antibody (CL488-12143) at dilution of 1:1000, CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated p63 Polyclonal antib (CL488-12143)

    IF Staining of A431 using CL488-12143

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CL488-12143 (p63 antibody) at dilution of 1:100.

  • Flow cytometry (FC) experiment of A431 cells using CoraLite® Plus 488-conjugated p63 Polyclonal antib (CL488-12143)

    FC experiment of A431 using CL488-12143

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human p63 (CL488-12143) (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-12143 targets p63 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen p63 fusion protein Ag2791 相同性解析による交差性が予測される生物種
Full Name tumor protein p63
Calculated molecular weight 680 aa, 77 kDa
GenBank accession numberBC039815
Gene symbol TP63
Gene ID (NCBI) 8626
RRIDAB_2883081
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TP63, also named KET, P63, P73H, P73L and TP73L, belongs to the p53 family. It is a homologue of the tumor suppressor p53 and p73 genes. It is involved in malignancy acquisition and maintenance of cells. Unlike p53, the p63 gene encodes multiple isotypes with remarkably divergent abilities to transactivate p53 reporter genes and induce apoptosis. TP63 acts as a sequence specific DNA binding transcriptional activator or repressor. This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 p63 antibody CL488-12143Download protocol
FC protocol for CL Plus 488 p63 antibody CL488-12143Download protocol
Standard Protocols
Click here to view our Standard Protocols