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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated p70(S6K) Polyclonal antibody

p70(S6K) Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-14485

Synonyms

Phospho-p70(S6K) (Thr389), RPS6KB1, p70 S6 kinase, p70 S6K, p70(S6K), p70S6K, p70-S6K 1, P70S6K1, p70 S6K 1, p70 S6K-alpha, S6K, S6K-beta-1, Serine/threonine-protein kinase 14A, Ribosomal protein S6 kinase beta-1, Ribosomal protein S6 kinase I, RPS6KB 1, 70 kDa ribosomal protein S6 kinase 1, EC:2.7.11.1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 647-conjugated p70(S6K) Polyclonal (CL647-14485)

    FC experiment of HeLa using CL647-14485

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human p70(S6K) (CL647-14485) (red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-14485 targets p70(S6K) in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen p70(S6K) fusion protein Ag5883 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S6 kinase, 70kDa, polypeptide 1
Calculated molecular weight 59 kDa
Observed molecular weight60-75 kDa
GenBank accession numberBC053365
Gene symbol p70 S6K
Gene ID (NCBI) 6198
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPS6KB1(Ribosomal protein S6 kinase beta-1) is also named as STK14A, p70 S6KA and belongs to the S6 kinase subfamily. RPS6KB1 is a major substrate of MTOR and acts as a crucial effector of MTOR signaling pathway. It plays a key role in cell growth and proliferation by regulating INS sensitivity, metabolism, protein synthesis, and cell cycle. RPS6KB1 may play an important role in the progression of HCC and could serve as a potential molecular target for HCC therapy (PMID:22684641). RPS6KB1 is a 70 kDa protein and has 5 isoforms with the calculated molecular mass of 51-59 kDa produced by alternative initiation.

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 p70(S6K) antibody CL647-14485Download protocol
Standard Protocols
Click here to view our Standard Protocols