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  • KD/KO Validated

pan Ras Monoclonal antibody, PBS Only (Capture/Detector)

pan Ras Monoclonal Antibody for WB, IHC, FC (Intra), IP, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit, chicken

Applications

WB, IHC, FC (Intra), IP, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4H4G7

Cat no : 60309-1-PBS

Synonyms

KRAS, KRAS4A, KRAS4B, EC:3.6.5.2, c-K-ras


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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60309-1-PBS targets pan Ras as part of a matched antibody pair:

MP50398-1: 60309-2-PBS capture and 60309-1-PBS detection (validated in Cytometric bead array)

MP80009-1: 60309-1-PBS capture and 81615-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat, pig, rabbit, chicken
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen pan Ras fusion protein Ag2700 相同性解析による交差性が予測される生物種
Full Name v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Calculated molecular weight 188 aa, 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC013572
Gene symbol KRAS
Gene ID (NCBI) 3845
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.