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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using pan Ras Monoclonal antibody (60309-1-Ig)

    WB analysis using 60309-1-Ig (same clone as 60309-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using pan Ras Monoclonal antibody (60309-1-Ig)

    WB analysis using 60309-1-Ig (same clone as 60309-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of ROS1728 cells using pan Ras Monoclonal antibody (60309-1-Ig)

    WB analysis of ROS1728 using 60309-1-Ig (same clone as 60309-1-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using pan Ras Monoclonal antibody (60309-1-Ig)

    WB analysis of HEK-293 using 60309-1-Ig (same clone as 60309-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Neuro-2a cells using pan Ras Monoclonal antibody (60309-1-Ig)

    WB analysis of Neuro-2a using 60309-1-Ig (same clone as 60309-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using pan Ras Monoclonal antibody (60309-1-Ig)

    IP experiment of mouse brain using 60309-1-Ig (same clone as 60309-1-PBS)

    IP result of anti-pan Ras (IP:60309-1-Ig, 5ug; Detection:60309-1-Ig 1:2000) with mouse brain tissue lysate 4000 ug. This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using pan Ras Monoclonal antibody (60309-1-Ig)

    IHC staining of human breast cancer using 60309-1-Ig (same clone as 60309-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using pan Ras Monoclonal antibody (60309-1-Ig)

    IHC staining of human breast cancer using 60309-1-Ig (same clone as 60309-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using pan Ras Monoclonal antibody (60309-1-Ig)

    IHC staining of human ovary cancer using 60309-1-Ig (same clone as 60309-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 60309-1-Ig (pan Ras antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using pan Ras Monoclonal antibody (60309-1-Ig)

    IHC staining of human lung cancer using 60309-1-Ig (same clone as 60309-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using pan Ras Monoclonal antibody (60309-1-Ig)

    IHC staining of human lung cancer using 60309-1-Ig (same clone as 60309-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using pan Ras Monoclonal antibody (60309-1-Ig)

    IHC staining of human colon cancer using 60309-1-Ig (same clone as 60309-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using pan Ras Monoclonal antibody (60309-1-Ig)

    IHC staining of human colon cancer using 60309-1-Ig (same clone as 60309-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using pan Ras Monoclonal antibody (60309-1-Ig)

    IF Staining of HEK-293 using 60309-1-Ig (same clone as 60309-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using pan Ras antibody (60309-1-Ig, Clone: 4H4G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using pan Ras Monoclonal antibody (60309-1-Ig)

    FC experiment of HeLa using 60309-1-Ig (same clone as 60309-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human pan Ras (60309-1-Ig, Clone:4H4G7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP80009-1

    Cytometric bead array standard curve of MP80009-1

    Cytometric bead array standard curve of MP80009-1, KRAS Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 60309-1-PBS. Detection antibody: 81615-1-PBS. Standard: Ag2700. Range: 0.391-50 ng/mL.

  • Cytometric bead array standard curve of MP50398-1

    Cytometric bead array standard curve of MP50398-1

    Cytometric bead array standard curve of MP50398-1, KRAS Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60309-2-PBS. Detection antibody: 60309-1-PBS. Standard:Ag2700. Range: 0.781-12.5 ng/mL.

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  • Featured Product
  • KD/KO Validated

pan Ras Monoclonal antibody, PBS Only (Capture/Detector)

pan Ras Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA
Cat No. 60309-1-PBS
Clone No.4H4G7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit, chicken

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA

Synonyms

KRAS, EC:3.6.5.2, c-K-ras, c-Ki-ras, c Ki ras

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60309-1-PBS targets pan Ras as part of a matched antibody pair:

MP50398-1: 60309-2-PBS capture and 60309-1-PBS detection (validated in Cytometric bead array)

MP80009-1: 60309-1-PBS capture and 81615-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat, pig, rabbit, chicken
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen pan Ras fusion protein Ag2700 相同性解析による交差性が予測される生物種
Full Name v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Calculated molecular weight 188 aa, 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC013572
Gene Symbol KRAS
Gene ID (NCBI) 3845
RRIDAB_2881422
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01116
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.