- Featured Product
- KD/KO Validated
pan Ras Monoclonal antibody, PBS Only (Capture/Detector)
pan Ras Monoclonal Antibody for WB, IHC, FC (Intra), IP, Cytometric bead array, Indirect ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig, rabbit, chicken
Applications
WB, IHC, FC (Intra), IP, Cytometric bead array, Indirect ELISA
Conjugate
Unconjugated
CloneNo.
4H4G7
Cat no : 60309-1-PBS
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
Application | Dilution |
---|---|
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. |
Product Information
60309-1-PBS targets pan Ras as part of a matched antibody pair:
MP50398-1: 60309-2-PBS capture and 60309-1-PBS detection (validated in Cytometric bead array)
MP80009-1: 60309-1-PBS capture and 81615-1-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
Tested Reactivity | human, mouse, rat, pig, rabbit, chicken |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | pan Ras fusion protein Ag2700 相同性解析による交差性が予測される生物種 |
Full Name | v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog |
Calculated molecular weight | 188 aa, 21 kDa |
Observed molecular weight | 21 kDa |
GenBank accession number | BC013572 |
Gene symbol | KRAS |
Gene ID (NCBI) | 3845 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. |
Background Information
The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.