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KD/KO Validated

pan Ras Monoclonal antibody

Name: pan Ras Monoclonal antibody
Brand: Proteintech
SKU: 60309-1-Ig

pan Ras Monoclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit, chicken

Applications

WB, IHC, FC (Intra), IP, ELISA, IF

Conjugate

Unconjugated

Publications

CloneNo.

4H4G7

Review Product

Cat no : 60309-1-Ig

Synonyms

KRAS, KRAS4A, KRAS4B, EC:3.6.5.2, c-K-ras

Validation Data Gallery

Filter:

AllWBIPIHCFC (Intra)

WB analysis using 60309-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
WB analysis using 60309-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 60309-1-Ig
HEK-293 cells were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
WB analysis of Neuro-2a using 60309-1-Ig
Neuro-2a cells were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
WB analysis of ROS1728 using 60309-1-Ig
ROS1728 cells were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 60309-1-Ig
IP result of anti-pan Ras (IP:60309-1-Ig, 5ug; Detection:60309-1-Ig 1:2000) with mouse brain tissue lysate 4000 ug.
IHC staining of human breast cancer using 60309-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 60309-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 60309-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 60309-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 60309-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 60309-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:50 (under 40x lens).
FC experiment of HeLa using 60309-1-Ig
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human pan Ras (60309-1-Ig, Clone:4H4G7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 60309-1-Ig

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	fetal human brain tissue, pig brain tissue, HEK-293 cells, Neuro-2a cells, ROS1728 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue, chicken brain tissue, human brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human breast cancer tissue, human colon cancer tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:20000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 13 publications below
IHC	See 4 publications below
IF	See 1 publications below

Product Information

60309-1-Ig targets pan Ras in WB, IHC, FC (Intra), IP, ELISA, IF applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.

Tested Reactivity	human, mouse, rat, pig, rabbit, chicken
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	pan Ras fusion protein Ag2700 相同性解析による交差性が予測される生物種
Full Name	v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Calculated molecular weight	188 aa, 21 kDa
Observed molecular weight	21 kDa
GenBank accession number	BC013572
Gene symbol	KRAS
Gene ID (NCBI)	3845
RRID	AB_2881422
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.

Protocols

Product Specific Protocols
WB protocol for pan Ras antibody 60309-1-Ig	Download protocol
IHC protocol for pan Ras antibody 60309-1-Ig	Download protocol
IP protocol for pan Ras antibody 60309-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Mol Cell TCR activation directly stimulates PYGB-dependent glycogenolysis to fuel the early recall response in CD8+ memory T cells. Authors - Huafeng Zhang View Article
human	WB	Cancer Res KRAS-NFκB-YY1-miR-489 signaling axis controls pancreatic cancer metastasis. Authors - Peng Yuan KD Validated View Article
human	IHC	Exp Mol Med Destabilization of β-catenin and RAS by targeting the Wnt/β-catenin pathway as a potential treatment for triple-negative breast cancer. Authors - Won-Ji Ryu View Article
human	WB	J Cell Mol Med DEPDC1 up-regulates RAS expression to inhibit autophagy in lung adenocarcinoma cells. Authors - Wei Wang View Article
human	WB	Mol Pharm Hyaluronic Acid-Modified Nanoparticles Self-Assembled from Linoleic Acid-Conjugated Chitosan for the Codelivery of miR34a and Doxorubicin in Resistant Breast Cancer Authors - Xiaoxia Yang View Article
human	WB	Oncol Rep EGF upregulates RFPL3 and hTERT via the MEK signaling pathway in non‑small cell lung cancer cells. Authors - Chuan Lin View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

pan Ras Monoclonal antibody

pan Ras Monoclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 60309-1-Ig

WB analysis using 60309-1-Ig

WB analysis of HEK-293 using 60309-1-Ig

WB analysis of Neuro-2a using 60309-1-Ig

WB analysis of ROS1728 using 60309-1-Ig

IP experiment of mouse brain using 60309-1-Ig

IHC staining of human breast cancer using 60309-1-Ig

IHC staining of human breast cancer using 60309-1-Ig

IHC staining of human lung cancer using 60309-1-Ig

IHC staining of human lung cancer using 60309-1-Ig

IHC staining of human colon cancer using 60309-1-Ig

IHC staining of human colon cancer using 60309-1-Ig

FC experiment of HeLa using 60309-1-Ig

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

TCR activation directly stimulates PYGB-dependent glycogenolysis to fuel the early recall response in CD8+ memory T cells.

KRAS-NFκB-YY1-miR-489 signaling axis controls pancreatic cancer metastasis.

Destabilization of β-catenin and RAS by targeting the Wnt/β-catenin pathway as a potential treatment for triple-negative breast cancer.

DEPDC1 up-regulates RAS expression to inhibit autophagy in lung adenocarcinoma cells.

Hyaluronic Acid-Modified Nanoparticles Self-Assembled from Linoleic Acid-Conjugated Chitosan for the Codelivery of miR34a and Doxorubicin in Resistant Breast Cancer

EGF upregulates RFPL3 and hTERT via the MEK signaling pathway in non‑small cell lung cancer cells.