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CoraLite®555-conjugated pan Ras Monoclonal antibody
pan Ras Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
FC (Intra)
Conjugate
CoraLite®555 Fluorescent Dye
CloneNo.
4H4G7
Cat no : CL555-60309
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL555-60309 targets pan Ras in FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | pan Ras fusion protein Ag2700 相同性解析による交差性が予測される生物種 |
| Full Name | v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog |
| Calculated molecular weight | 188 aa, 21 kDa |
| Observed molecular weight | 21 kDa |
| GenBank accession number | BC013572 |
| Gene symbol | KRAS |
| Gene ID (NCBI) | 3845 |
| RRID | AB_2919662 |
| Conjugate | CoraLite®555 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 557 nm / 570 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL555 pan Ras antibody CL555-60309 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
