CoraLite® Plus 488-conjugated pan-keratin Polyclonal antibody
pan-keratin Polyclonal Antibody for IF/ICC, IF-P, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC, IF-P, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-26411
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF-P detected in | human colon cancer tissue, human lung cancer tissue |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-26411 targets pan-keratin in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | pan-keratin fusion protein Ag24184 相同性解析による交差性が予測される生物種 |
| Full Name | keratin 5 |
| Calculated molecular weight | 590 aa, 62 kDa |
| GenBank accession number | BC024292 |
| Gene symbol | Cytokeratin 5 |
| Gene ID (NCBI) | 3852 |
| RRID | AB_2919208 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers. Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases. This antibody is a pan-keratin antibody.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 pan-keratin antibody CL488-26411 | Download protocol |
| FC protocol for CL Plus 488 pan-keratin antibody CL488-26411 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
