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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using peroxiredoxin 2 Polyclonal antibody (10545-2-AP)

    WB analysis using 10545-2-AP (same clone as 10545-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10545-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using peroxiredoxin 2 Polyclonal antibody (10545-2-AP)

    WB analysis of HepG2 using 10545-2-AP (same clone as 10545-2-PBS)

    WB result of peroxiredoxin 2 antibody (10545-2-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-peroxiredoxin 2 transfected HepG2 cells. This data was developed using the same antibody clone with 10545-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using peroxiredoxin 2 Polyclonal antibody (10545-2-AP)

    IP experiment of HepG2 using 10545-2-AP (same clone as 10545-2-PBS)

    IP result of anti-peroxiredoxin 2 (IP:10545-2-AP, 4ug; Detection:10545-2-AP 1:4000) with HepG2 cells lysate 960 ug. This data was developed using the same antibody clone with 10545-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using peroxiredoxin 2 Polyclonal antibody (10545-2-AP)

    IHC staining of human liver using 10545-2-AP (same clone as 10545-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10545-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using peroxiredoxin 2 Polyclonal antibody (10545-2-AP)

    IHC staining of human liver using 10545-2-AP (same clone as 10545-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10545-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using peroxiredoxin 2 Polyclonal antibody (10545-2-AP)

    IHC staining of human hepatocirrhosis using 10545-2-AP (same clone as 10545-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:600 (under 10x lens). This data was developed using the same antibody clone with 10545-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using peroxiredoxin 2 Polyclonal antibody (10545-2-AP)

    IHC staining of human hepatocirrhosis using 10545-2-AP (same clone as 10545-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:600 (under 40x lens). This data was developed using the same antibody clone with 10545-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using peroxiredoxin 2 Polyclonal antibody (10545-2-AP)

    IF Staining of HepG2 using 10545-2-AP (same clone as 10545-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using peroxiredoxin 2 antibody (10545-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 10545-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using peroxiredoxin 2 Polyclonal antibody (10545-2-AP)

    IF Staining of HeLa using 10545-2-AP (same clone as 10545-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using peroxiredoxin 2 antibody (10545-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10545-2-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

peroxiredoxin 2 Polyclonal antibody, PBS Only

peroxiredoxin 2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10545-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

PRDX2, Peroxiredoxin-2, NKEF-B, NKEFB, Natural killer cell-enhancing factor B

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10545-2-PBS targets peroxiredoxin 2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen peroxiredoxin 2 fusion protein Ag0835 相同性解析による交差性が予測される生物種
Full Name peroxiredoxin 2
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC003022
Gene Symbol peroxiredoxin 2
Gene ID (NCBI) 7001
RRIDAB_2168202
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP32119
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PRDX2, also named as TSA, PRP, NKEFB and TDPX1, belongs to the ahpC/TSA family. It is known to act as an antioxidant enzyme whose main function is H2O2 reduction in cells. PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. It may play an important role in eliminating peroxides generated during metabolism. PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. PRDX2 actions may be related to the expression of NFKB and IKB.(PMID:21248284)