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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using peroxiredoxin 2 Polyclonal antibody (20569-1-AP)

    WB analysis of HeLa using 20569-1-AP (same clone as 20569-1-PBS)

    WB result of peroxiredoxin 2 antibody (20569-1-AP, 1:5000) with si-Control and si-peroxiredoxin 2 transfected HeLa cells. This data was developed using the same antibody clone with 20569-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using peroxiredoxin 2 Polyclonal antibody (20569-1-AP)

    WB analysis of HeLa using 20569-1-AP (same clone as 20569-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20569-1-AP (peroxiredoxin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20569-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using peroxiredoxin 2 Polyclonal antibody (20569-1-AP)

    WB analysis of HeLa using 20569-1-AP (same clone as 20569-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20569-1-AP (peroxiredoxin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20569-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using peroxiredoxin 2 Polyclonal antibody (20569-1-AP)

    IF Staining of HepG2 using 20569-1-AP (same clone as 20569-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using PRDX2 antibody 20569-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20569-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

peroxiredoxin 2 Polyclonal antibody, PBS Only

peroxiredoxin 2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 20569-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

PRDX2, Peroxiredoxin-2, NKEF-B, NKEFB, Natural killer cell-enhancing factor B

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20569-1-PBS targets peroxiredoxin 2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name peroxiredoxin 2
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberNM_005809
Gene Symbol peroxiredoxin 2
Gene ID (NCBI) 7001
RRIDAB_10695186
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP32119
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PRDX2, also named as NKEFB, TDPX1, TSA and PRP, belongs to the ahpC/TSA family. It is involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. PRDX2 may play an important role in eliminating peroxides generated during metabolism. It might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. The antibody is specific to PRDX2.