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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated smooth muscle actin specific Polyclonal antibody

smooth muscle actin specific Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-55135

Synonyms

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, Alpha actin 2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using CoraLite® Plus 488-conjugated smooth muscle actin (CL488-55135)

    IF Staining of mouse heart using CL488-55135

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488 smooth muscle actin-specific antibody (CL488-55135) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CoraLite® Plus 488-conjugated smooth muscle actin (CL488-55135)

    IF Staining of mouse spleen using CL488-55135

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite® Plus 488 smooth muscle actin specific antibody (CL488-55135) at dilution of 1:200, F4/80 antibody (28463-1-AP, Magenta).

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Tested Applications

Positive IF-P detected inmouse heart tissue, mouse spleen tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-55135 targets smooth muscle actin specific in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name actin, alpha 2, smooth muscle, aorta
Calculated molecular weight 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberNM_001613
Gene symbol Alpha smooth muscle actin
Gene ID (NCBI) 59
RRIDAB_2919221
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACTA2, also named as a-SMA,AAT6;ACTSA belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6). The immunogen of this antibody is a specific domain of ACTA2. This antibody stains the aorta only.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 smooth muscle actin specific antibody CL488-55135Download protocol
Standard Protocols
Click here to view our Standard Protocols