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  • Featured Product
  • KD/KO Validated

Biotin-conjugated t-Plasminogen activator/tPA Polyclonal antibody

t-Plasminogen activator/tPA Polyclonal Antibody for IHC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC

Conjugate

Biotin

Cat no : Biotin-10147

Synonyms

PLAT, tPA, T PA, Reteplase, EC:3.4.21.68

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using Biotin-conjugated tPA Polyclonal antibody (Biotin-10147)

    IHC staining of mouse brain using Biotin-10147

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-10147 (tPA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Biotin-conjugated tPA Polyclonal antibody (Biotin-10147)

    IHC staining of mouse brain using Biotin-10147

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-10147 (tPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Biotin-conjugated tPA Polyclonal antibody (Biotin-10147)

    IHC staining of human kidney using Biotin-10147

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using Biotin-10147 (tPA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Biotin-conjugated tPA Polyclonal antibody (Biotin-10147)

    IHC staining of human kidney using Biotin-10147

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using Biotin-10147 (tPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inmouse brain tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-10147 targets t-Plasminogen activator/tPA in IHC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen t-Plasminogen activator/tPA fusion protein Ag0200 相同性解析による交差性が予測される生物種
Full Name plasminogen activator, tissue
Calculated molecular weight 57 kDa
Observed molecular weight 32-35 kDa, 65 kDa
GenBank accession numberBC002795
Gene symbol tPA
Gene ID (NCBI) 5327
Conjugate Biotin
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Plasminogen activator, tissue (PLAT, synonyms: TPA, T-PA) is a tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein (33 kDa and 32 kDa). PLAT enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. tPA has 4 isoforms produced by alternative splicing with the MW of 63 kDa, 33 kDa, 57 kDa and 44 kDa.

Protocols

Product Specific Protocols
IHC protocol for Biotin t-Plasminogen activator/tPA antibody Biotin-10147Download protocol
Standard Protocols
Click here to view our Standard Protocols