Validation Data Gallery
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Tested Applications
| Positive IF-P detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-60213 targets transgelin/SM22 in IF-P applications and shows reactivity with human, rat, pig, mouse samples.
| Tested Reactivity | human, rat, pig, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | transgelin/SM22 fusion protein Ag0764 相同性解析による交差性が予測される生物種 |
| Full Name | transgelin |
| Calculated molecular weight | 22 kDa |
| Observed molecular weight | 18+22 kDa |
| GenBank accession number | BC004927 |
| Gene Symbol | transgelin/SM22 |
| Gene ID (NCBI) | 6876 |
| RRID | AB_2883444 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q01995 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 transgelin/SM22 antibody CL594-60213 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |