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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated transgelin/SM22 Monoclonal antibody

transgelin/SM22 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, mouse

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2A10C2

Cat no : CL594-60213

Synonyms

22 kDa actin binding protein, DKFZp686P11128, Protein WS3 10, SM22, SM22 alpha, SM22alpha, SMCC, Smooth muscle protein 22 alpha, TAGLN, TAGLN1, transgelin, TRANSGELIN 1, transgelin/SM22, WS3 10

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using CoraLite®594-conjugated transgelin/SM22 Monoclonal (CL594-60213)

    IF Staining of mouse heart using CL594-60213

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CL594-60213 (transgelin/SM22 antibody) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using CoraLite®594-conjugated transgelin/SM22 Monoclonal (CL594-60213)

    IF Staining of mouse heart using CL594-60213

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CL594-60213 (transgelin/SM22 antibody) at dilution of 1:100.

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Tested Applications

Positive IF-P detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL594-60213 is transgelin/SM22 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, pig, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen transgelin/SM22 fusion protein Ag0764 相同性解析による交差性が予測される生物種
Full Name transgelin
Calculated molecular weight 22 kDa
Observed molecular weight 18+22 kDa
GenBank accession numberBC004927
Gene symbol transgelin/SM22
Gene ID (NCBI) 6876
RRIDAB_2883444
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells.

Protocols

Product Specific Protocols
IF protocol for CL594 transgelin/SM22 antibody CL594-60213Download protocol
Standard Protocols
Click here to view our Standard Protocols