• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

uPAR/CD87 Recombinant antibody

uPAR/CD87 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230148B10

Cat no : 82953-2-RR

Synonyms

uPAR, PLAUR, PLAUR, UPAR, U PAR, MO3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using uPAR/CD87 Recombinant antibody (82953-2-RR)

    WB analysis using 82953-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82953-2-RR (uPAR/CD87 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using uPAR/CD87 Recombinant antibody (82953-2-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 82953-2-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82953-2-RR (uPAR/CD87 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using uPAR/CD87 Recombinant antibody (82953-2-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 82953-2-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82953-2-RR (uPAR/CD87 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using uPAR/CD87 Recombinant antibody (82953-2-RR)

    IHC staining of mouse spleen using 82953-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82953-2-RR (uPAR/CD87 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using uPAR/CD87 Recombinant antibody (82953-2-RR)

    IHC staining of mouse spleen using 82953-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82953-2-RR (uPAR/CD87 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82953-2-RR

    Affinity and Kinetic Characterization of 82953-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82953-2-RR against Human uPAR, PLAUR were performed. The affinity constant is 0.527 nM.

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inHeLa cells, DU 145 cells, THP-1 cells, Saos-2 cells, PC-3 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82953-2-RR targets uPAR/CD87 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen uPAR/CD87 fusion protein Ag28056 相同性解析による交差性が予測される生物種
Full Name PLAUR
Calculated molecular weight 37 kDa
Observed molecular weight35-50 kDa
GenBank accession numberBC002788
Gene symbol uPAR
Gene ID (NCBI) 5329
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

uPAR is a highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodeling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)

Protocols

Product Specific Protocols
WB protocol for uPAR/CD87 antibody 82953-2-RRDownload protocol
IHC protocol for uPAR/CD87 antibody 82953-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols