Various lysates were subjected to SDS PAGE followed by western blot with 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
1X10^6 SH-SY5Y cells were stained with 0.2ug NCAM1/CD56 antibody (14255-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Immunohistochemical analysis of paraffin-embedded human small cell lung carcinoma tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:16000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human small cell lung carcinoma slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:16000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
