G17038-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.
G17038-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.
PMA, LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 27095-1-AP (CXCL8/IL-8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
PMA, LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 17038-1-AP (CXCL8/IL-8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17038-1-AP (CXCL8/IL-8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17038-1-AP (CXCL8/IL-8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Purity of recombinant human IL-8 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue
Recombinant human IL-8 (HZ-1318) stimulates dose-dependent proliferation of the THP-1 human monocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. THP-1 cells were treated with increasing concentrations of recombinant IL-8 for 144 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 100-500 ng/mL.

Proteintech IL-8 (HZ-1318) demonstrates a 2.5-fold decrease in EC50 and a greater fold induction compared to leading competitors. Recombinant human IL-8 stimulates dose-dependent proliferation of the THP-1 human monocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. THP-1 cells were treated with increasing concentrations of recombinant IL-8 for 144 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 100-500 ng/mL.