1X10^6 BALB/c mouse splenocytes were surface stained with CoraLite®488 Anti-Mouse CD3 (CL488-65077, Clone: 17A2) and 0.25 ug CoraLite® Plus 647 Anti-Mouse MHC Class II (I-A/I-E) (CL647-65122, Clone: M5/114.15.2) or CoraLite® Plus 647-Conjugated rat IgG2b isotype control. Cells were not fixed.