1X10^6 A375 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human S100 Beta (CL488-15146) (red), or 0.4 ug CoraLite®488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).