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ASGR1 Polyclonal antibody
ASGR1 Polyclonal Antibody for WB, IHC, IF-P, FC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF-P, FC, IP, CoIP, ELISA, Blocking assay
Conjugate
Unconjugated
Cat no : 11739-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, human liver tissue, mouse liver tissue, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | rat liver tissue, human liver cancer tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human liver cancer tissue, human liver tissue, rat liver tissue |
| Positive FC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 11 publications below |
| IHC | See 2 publications below |
| IF | See 5 publications below |
| FC | See 4 publications below |
| CoIP | See 1 publications below |
Product Information
11739-1-AP targets ASGR1 in WB, IHC, IF-P, FC, IP, CoIP, ELISA, Blocking assay applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ASGR1 fusion protein Ag2310 相同性解析による交差性が予測される生物種 |
| Full Name | asialoglycoprotein receptor 1 |
| Calculated molecular weight | 291 aa, 33 kDa |
| Observed molecular weight | 38-42 kDa |
| GenBank accession number | BC032130 |
| Gene symbol | ASGR1 |
| Gene ID (NCBI) | 432 |
| RRID | AB_2059675 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ASGR1 antibody 11739-1-AP | Download protocol |
| IHC protocol for ASGR1 antibody 11739-1-AP | Download protocol |
| IF protocol for ASGR1 antibody 11739-1-AP | Download protocol |
| IP protocol for ASGR1 antibody 11739-1-AP | Download protocol |
| FC protocol for ASGR1 antibody 11739-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nano Lett A Potent Branched-Tail Lipid Nanoparticle Enables Multiplexed mRNA Delivery and Gene Editing In Vivo. | ||
Acta Biomater Modeling nonalcoholic fatty liver disease on a liver lobule chip with dual blood supply. | ||
Biomaterials Dual targeted delivery of statins and nucleic acids by chitosan-based nanoparticles for enhanced antiatherosclerotic efficacy. | ||
Cancer Res ASO-Based PKM Splice-Switching Therapy Inhibits Hepatocellular Carcinoma Growth. | ||
Mol Ther Enhanced Potency of GalNAc-Conjugated Antisense Oligonucleotides in Hepatocellular Cancer Models. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kamal (Verified Customer) (02-15-2024) | Mouse liver lysates were subjected to SDS PAGE followed by western blot with 11739-1-AP (ASGR1 antibody) at dilution of 1:25000 incubated at 4 degree C overnight. ASGR1 bands appeared at about 42 kDa.
|
FH Kamal (Verified Customer) (02-15-2024) | Mouse liver lysates were subjected to SDS PAGE followed by western blot with 11739-1-AP (ASGR1 antibody) at dilution of 1:25000 incubated at 4 degree C overnight. ASGR1 bands appeared at about 42 kDa.
|
FH James (Verified Customer) (11-18-2022) | Very good antibody! Used it to label hepatocytes in section of paraffin-embedded liver tissue
 |
