- Featured Product
- KD/KO Validated
CoraLite® Plus 488-conjugated ASGR1 Polyclonal antibody
ASGR1 Polyclonal Antibody for IF-P, FC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF-P, FC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-11739
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF-P detected in | rat liver tissue, human liver cancer tissue |
| Positive FC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
CL488-11739 targets ASGR1 in IF-P, FC applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ASGR1 fusion protein Ag2310 相同性解析による交差性が予測される生物種 |
| Full Name | asialoglycoprotein receptor 1 |
| Calculated molecular weight | 291 aa, 33 kDa |
| GenBank accession number | BC032130 |
| Gene symbol | ASGR1 |
| Gene ID (NCBI) | 432 |
| RRID | AB_2883080 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery. This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ASGR1 antibody CL488-11739 | Download protocol |
| FC protocol for CL Plus 488 ASGR1 antibody CL488-11739 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
MedComm (2020) LILAR, a novel long noncoding RNA regulating autophagy in the liver tissues of endotoxemic mice through a competing endogenous RNA mechanism |
