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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DOHH Monoclonal antibody (68151-1-Ig)

    WB analysis using 68151-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68151-1-Ig (DOHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of various lysates using DOHH Monoclonal antibody (68151-1-Ig)

    WB analysis using 68151-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68151-1-Ig (DOHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of LNCaP cells using DOHH Monoclonal antibody (68151-1-Ig)

    IF Staining of LNCaP using 68151-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using DOHH antibody (68151-1-Ig, Clone: 1H5H7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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DOHH Monoclonal antibody

DOHH Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68151-1-Ig
Clone No.1H5H7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

HLRC1, HEAT-like repeat-containing protein 1, hDOHH, EC:1.14.99.29, 1H5H7

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, HSC-T6 cells, HeLa cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells
Positive IF/ICC detected inLNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68151-1-Ig targets DOHH in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DOHH fusion protein Ag13041 相同性解析による交差性が予測される生物種
Full Name deoxyhypusine hydroxylase/monooxygenase
Calculated molecular weight 33 kDa
Observed molecular weight30-35 kDa
GenBank accession numberBC002817
Gene Symbol DOHH
Gene ID (NCBI) 83475
RRIDAB_2935259
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BU89
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Deoxyhypusine hydroxylase (DOHH) is the enzyme catalyzing the second step in the post-translational synthesis of hypusine [Nε-(4-amino-2-hydroxybutyl)lysine] in the eukaryotic initiation factor 5A (eIF5A). DOHH has been reported to mediate several crucial cellular functions, including cellular proliferation, differentiation and apoptosis. Moreover, previous studies have established that DOHH is highly involved in several essential biological processes driving human diseases including cancer growth, malarial drug resistance, and HIV-1 replication. In particular, the DOHH/eIF5A signaling pathway was revealed to mediate the ability of nerve growth factor to enhance neuronal growth and survival, highlighting the importance of DOHH in neuroprotection(PMID: 35007708, PMID: 16371467, PMID: 22908221, PMID: 19706422).

Protocols

Product Specific Protocols
WB protocol for DOHH antibody 68151-1-IgDownload protocol
IF protocol for DOHH antibody 68151-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols