• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PFKP Monoclonal antibody (68129-1-Ig)

    WB analysis using 68129-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68129-1-Ig (PFKP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of various lysates using PFKP Monoclonal antibody (68129-1-Ig)

    WB analysis using 68129-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68129-1-Ig (PFKP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PFKP Monoclonal antibody (68129-1-Ig)

    IF Staining of HeLa using 68129-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PFKP antibody (68129-1-Ig, Clone: 1H4D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using PFKP Monoclonal antibody (68129-1-Ig)

    IF Staining of BxPC-3 using 68129-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using PFKP antibody (68129-1-Ig, Clone: 1H4D4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

PFKP Monoclonal antibody

PFKP Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68129-1-Ig
Clone No.1H4D4
Publications(2)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit

Applications

WB, IF/ICC, ELISA

Synonyms

1H4D4, 6-phosphofructokinase type C, ATP-dependent 6-phosphofructokinase, platelet type, ATP-PFK, EC:2.7.1.11

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, rabbit brain tissue, rat brain tissue, mouse brain tissue, A549 cells, MCF-7 cells, HEK-293 cells, HeLa cells, Jurkat cells
Positive IF/ICC detected inHeLa cells, BxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

68129-1-Ig targets PFKP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Reactivity human, mouse, rat, rabbit
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PFKP fusion protein Ag25206 相同性解析による交差性が予測される生物種
Full Name phosphofructokinase, platelet
Calculated molecular weight 784 aa, 86 kDa
Observed molecular weight80-86 kDa
GenBank accession numberBC029138
Gene Symbol PFKP
Gene ID (NCBI) 5214
RRIDAB_2923656
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ01813
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PFKP(6-phosphofructokinase, platelet type) is also named as PFKF, PFK-C and belongs to the phosphofructokinase family. This form of PFK is best called the 'platelet' type and symbolized PFKP because it is the only form made by platelets, whereas fibroblasts have more than one form of PFK (Francke (1983)). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The observed molecular weight of PFKP monomer is 80-86 kDa. The molecular mass of PFKP dimer is 171 kDa (PMID: 28607489).

Protocols

Product Specific Protocols
WB protocol for PFKP antibody 68129-1-IgDownload protocol
IF protocol for PFKP antibody 68129-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

medRxiv

Depressed Proximal Glycolysis in Myocardium Of Human Heart Failure with Preserved Ejection Fraction

Authors - Navid Koleini
View Article
mouseWB

Metabolism

Protein kinase N1 deficiency results in upregulation of cerebral energy metabolism and is highly protective in in vivo and in vitro stroke models

Authors - Stephanie Zur Nedden
View Article