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  • Featured Product
  • KD/KO Validated

SYK Monoclonal antibody

SYK Monoclonal Antibody for WB, IHC, IF-P, IP, ELISA
Cat No. 66721-1-Ig
Clone No.4C4A12

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (2)

Applications

WB, IHC, IF-P, IP, ELISA

Tyrosine protein kinase SYK, spleen tyrosine kinase, p72-Syk, IMD82, EC:2.7.10.2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inRaji cells, THP-1 cells, Ramos cells, Daudi cells
Positive IP detected inMDA-MB-231 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66721-1-Ig targets SYK in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SYK fusion protein Ag17654 相同性解析による交差性が予測される生物種
Full Name spleen tyrosine kinase
Calculated molecular weight 635 aa, 72 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC001645
Gene Symbol SYK
Gene ID (NCBI) 6850
ENSEMBL Gene IDENSG00000165025
RRIDAB_2882072
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP43405
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. Although SYK has not been linked to a human disease, defective expression of the closely related T-cell tyrosine kinase ZAP-70 has been associated with severe combined immunodeficiency(PMID:11494125). This protein has 2 isoforms produced by alternative splicing. SYK also promotes liver fibrosis by activation of hepatic stellate cells and acts as a biomarker for human hepatocellular carcinoma. (PMID: 29537660)

Protocols

Product Specific Protocols
WB protocol for SYK antibody 66721-1-IgDownload protocol
IHC protocol for SYK antibody 66721-1-IgDownload protocol
IF protocol for SYK antibody 66721-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cancer Lett

SIGLEC15 amplifies immunosuppressive properties of tumor-associated macrophages in pancreatic cancer.

Authors - Tian-Jiao Li
humanWB

Exp Cell Res

CD93 promotes acute myeloid leukemia development and is a potential therapeutic target

Authors - Jie Jia
mouseWB,IF

Cell Rep

Podocyte SIRPα reduction aggravates lupus nephritis via promoting T cell inflammatory responses

Authors - Bin Qian
mouseWB

Cancer Cell

Distinct roles of TREM2 in central nervous system cancers and peripheral cancers

Authors - Jian Zhong
humanWB,IP,IF

F1000Res

Identification of high-performing antibodies for tyrosine-protein kinase SYK for use in Western Blot, immunoprecipitation and immunofluorescence

Authors - Walaa Alshafie
  • KO Validated
mouse,pigWB

PLoS Pathog

Pseudorabies Virus UL4 protein promotes the ASC-dependent inflammasome activation and pyroptosis to exacerbate inflammation

Authors - Xiaohua Zhang
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