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KD/KO Validated

TRIM24 Polyclonal antibody

Name: TRIM24 Polyclonal antibody
Brand: Proteintech
SKU: 14208-1-AP
Rating: 5.00 (1 reviews)

TRIM24 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 14208-1-AP

Synonyms

TIF1-alpha, TIF1A, TIF1, RING-type E3 ubiquitin transferase TIF1-alpha, EC:2.3.2.27

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HeLa using 14208-1-AP
WB result of TRIM24 antibody (14208-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIM24 transfected HeLa cells.
WB analysis using 14208-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 14208-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of BGC-823 using 14208-1-AP
BGC-823 cells were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14208-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 Antibody) at dilution of 1:1500 incubated at 4 degree celsius over night.
WB analysis of L02 using 14208-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 14208-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 14208-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 14208-1-AP
IP result of anti-TRIM24 (IP:14208-1-AP, 4ug; Detection:14208-1-AP 1:6000) with HeLa cells lysate 1085 ug.
IHC staining of human prostate cancer using 14208-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14208-1-AP (TRIM24 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human prostate cancer using 14208-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14208-1-AP (TRIM24 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HeLa using 14208-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRIM24 antibody (14208-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Cat No. 14208-1-AP

Size/Concentration

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, L02 cells, BGC-823 cells, human heart tissue, mouse skeletal muscle tissue, MCF-7 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 13 publications below
WB	See 30 publications below
IHC	See 17 publications below
IF	See 6 publications below
IP	See 5 publications below
CoIP	See 1 publications below
ChIP	See 5 publications below

Product Information

14208-1-AP targets TRIM24 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TRIM24 fusion protein Ag5417 相同性解析による交差性が予測される生物種
Full Name	tripartite motif-containing 24
Calculated molecular weight	117 kDa
Observed molecular weight	117 kDa
GenBank accession number	BC028689
Gene symbol	TRIM24
Gene ID (NCBI)	8805
RRID	AB_2256646
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TRIM24, also named as RNF82, TIF1 and TIF1A, interacts selectively in vitro with the AF2-activating domain of the estrogen receptors. Association with DNA-bound estrogen receptors, it requires the presence of estradiol. It is a regulation protein of P53. Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) wich is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue.

Protocols

Product Specific Protocols
WB protocol for TRIM24 antibody 14208-1-AP	Download protocol
IHC protocol for TRIM24 antibody 14208-1-AP	Download protocol
IF protocol for TRIM24 antibody 14208-1-AP	Download protocol
IP protocol for TRIM24 antibody 14208-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Nature TRIM24 links a non-canonical histone signature to breast cancer. Authors - Tsai Wen-Wei WW View Article
human	WB,CoIP	Mol Cell p53 engages the cGAS/STING cytosolic DNA sensing pathway for tumor suppression Authors - Monisankar Ghosh KD Validated View Article
human	WB	Mol Cell TRIP12 promotes small-molecule-induced degradation through K29/K48-branched ubiquitin chains. Authors - Ai Kaiho-Soma View Article
human	WB	Nat Chem Biol Functional TRIM24 degrader via conjugation of ineffectual bromodomain and VHL ligands. Authors - Lara N Gechijian View Article
human	WB, IP, IHC	Nat Commun TRIM24 is an oncogenic transcriptional co-activator of STAT3 in glioblastoma. Authors - Deguan Lv View Article
human	WB,IF	Nat Commun Identification of proximal SUMO-dependent interactors using SUMO-ID. Authors - Orhi Barroso-Gomila View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Parijat (Verified Customer) (09-20-2023)

Good antibody for Western

Applications: Western Blot

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRIM24 Polyclonal antibody

TRIM24 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HeLa using 14208-1-AP

WB analysis using 14208-1-AP

WB analysis using 14208-1-AP

WB analysis of BGC-823 using 14208-1-AP

WB analysis of HeLa using 14208-1-AP

WB analysis of L02 using 14208-1-AP

WB analysis of human heart using 14208-1-AP

WB analysis of mouse skeletal muscle using 14208-1-AP

IP experiment of HeLa using 14208-1-AP

IHC staining of human prostate cancer using 14208-1-AP

IHC staining of human prostate cancer using 14208-1-AP

IF Staining of HeLa using 14208-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

TRIM24 links a non-canonical histone signature to breast cancer.

p53 engages the cGAS/STING cytosolic DNA sensing pathway for tumor suppression

TRIP12 promotes small-molecule-induced degradation through K29/K48-branched ubiquitin chains.

Functional TRIM24 degrader via conjugation of ineffectual bromodomain and VHL ligands.

TRIM24 is an oncogenic transcriptional co-activator of STAT3 in glioblastoma.

Identification of proximal SUMO-dependent interactors using SUMO-ID.

Reviews

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