VDAC3 Polyclonal antibody

VDAC3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14451-1-AP

Synonyms

HD VDAC3, hVDAC3, VDAC 3, VDAC3



Tested Applications

Positive WB detected inmouse heart tissue, human brain tissue, human heart tissue, mouse brain tissue
Positive IHC detected inhuman liver cancer tissue, human osteosarcoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14451-1-AP targets VDAC3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VDAC3 fusion protein Ag5960
Full Name voltage-dependent anion channel 3
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC056870
Gene Symbol VDAC3
Gene ID (NCBI) 7419
RRIDAB_2212351
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VDAC3 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules.

Protocols

Product Specific Protocols
WB protocol for VDAC3 antibody 14451-1-APDownload protocol
IHC protocol for VDAC3 antibody 14451-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Authors - Pengli Zheng
mouseWB

Mol Ther Nucleic Acids

Salvia miltiorrhiza-Derived Sal-miR-58 Induces Autophagy and Attenuates Inflammation in Vascular Smooth Muscle Cells.

Authors - Yan Qin
humanIHC

Oncotarget

Proteomics-based identification of VDAC1 as a tumor promoter in cervical carcinoma.

Authors - Changlin Zhang
WB

J Cell Sci

Doublet 7 shortening, doublet 5-preferential poly-Glu reduction, and beating stall of sperm flagella in Ttll9-/- mice.

Authors - Alu Konno
ratWB

Int J Mol Med

Herceptin induces ferroptosis and mitochondrial dysfunction in H9c2 cells.

Authors - Lei Sun
humanWB

Front Aging Neurosci

The Protective Role of Mitochondrial Ferritin on Erastin-Induced Ferroptosis.

Authors - Yue-Qi Wang