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ACAT2 Polyclonal antibody
ACAT2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Conjugate
Unconjugated
Cat no : 14755-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | Caco-2 cells, HepG2 cells, K-562 cells, MOLT-4 cells, mouse liver, rat liver |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human liver tissue, human lung tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IHC | See 3 publications below |
Product Information
14755-1-AP targets ACAT2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ACAT2 fusion protein Ag6503 相同性解析による交差性が予測される生物種 |
| Full Name | acetyl-Coenzyme A acetyltransferase 2 |
| Calculated molecular weight | 41 kDa |
| Observed molecular weight | 40-42 kDa |
| GenBank accession number | BC000408 |
| Gene symbol | ACAT2 |
| Gene ID (NCBI) | 39 |
| RRID | AB_2220236 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ACAT2 antibody 14755-1-AP | Download protocol |
| IHC protocol for ACAT2 antibody 14755-1-AP | Download protocol |
| IF protocol for ACAT2 antibody 14755-1-AP | Download protocol |
| IP protocol for ACAT2 antibody 14755-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Res Blocking FSH inhibits hepatic cholesterol biosynthesis and reduces serum cholesterol. | ||
Cell Res Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress. | ||
Cancer Cell Int Polyamine metabolism patterns characterized tumor microenvironment, prognosis, and response to immunotherapy in colorectal cancer | ||
iScience Hepatocyte activity of the cholesterol sensor smoothened regulates cholesterol and bile acid homeostasis in mice. | ||
J Agric Food Chem Oral Administration of Succinoglycan Riclin Improves Diet-Induced Hypercholesterolemia in Mice. | ||
Food Res Int Wild Lonicera caerulea berry polyphenol extract reduces cholesterol accumulation and enhances antioxidant capacity in vitro and in vivo. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Sarah-Eve (Verified Customer) (03-01-2023) | Very good working on PVDF membrane, blocking solution milk 5%
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