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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated ACAT2 Polyclonal antibody

ACAT2 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-14755

Synonyms

EC:2.3.1.9, Cytosolic acetoacetyl-CoA thiolase, ACTL, Acetyl-CoA transferase-like protein, Acetyl-CoA acetyltransferase, cytosolic

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 647-conjugated ACAT2 Polyclonal ant (CL647-14755)

    FC experiment of HepG2 using CL647-14755

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human ACAT2 (CL647-14755) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-14755 targets ACAT2 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACAT2 fusion protein Ag6503 相同性解析による交差性が予測される生物種
Full Name acetyl-Coenzyme A acetyltransferase 2
Calculated molecular weight 41 kDa
Observed molecular weight 40-42 kDa
GenBank accession numberBC000408
Gene symbol ACAT2
Gene ID (NCBI) 39
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Acyl-CoA: cholesterolacyltransferase (ACAT) is an in tracellular membrane-bound enzyme that uses cholesterol and long chain fatty acyl-coenzyme A (CoA) as substrates to produce cholesteryl esters. Two isoforms of the enzyme ACAT, i.e., ACAT1 and ACAT2, are known to exist. ACAT2, which is expressed in liver and intestine cells, has been found to play an important role in intracellular cholesterol homeostasis and univariate survival analyses have shown that ACAT2 expression is associated with a poor clear cell renal cell carcinoma associated survival.

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 ACAT2 antibody CL647-14755Download protocol
Standard Protocols
Click here to view our Standard Protocols