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CoraLite® Plus 555-conjugated ACAT2 Polyclonal antibody
ACAT2 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 555 Fluorescent Dye
Cat no : CL555-14755
Synonyms
Validation Data Gallery
Tested Applications
Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL555-14755 targets ACAT2 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ACAT2 fusion protein Ag6503 相同性解析による交差性が予測される生物種 |
Full Name | acetyl-Coenzyme A acetyltransferase 2 |
Calculated molecular weight | 41 kDa |
Observed molecular weight | 40-42 kDa |
GenBank accession number | BC000408 |
Gene symbol | ACAT2 |
Gene ID (NCBI) | 39 |
Conjugate | CoraLite® Plus 555 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 554 nm / 570 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Acyl-CoA: cholesterolacyltransferase (ACAT) is an in tracellular membrane-bound enzyme that uses cholesterol and long chain fatty acyl-coenzyme A (CoA) as substrates to produce cholesteryl esters. Two isoforms of the enzyme ACAT, i.e., ACAT1 and ACAT2, are known to exist. ACAT2, which is expressed in liver and intestine cells, has been found to play an important role in intracellular cholesterol homeostasis and univariate survival analyses have shown that ACAT2 expression is associated with a poor clear cell renal cell carcinoma associated survival.
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 555 ACAT2 antibody CL555-14755 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |