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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®555-conjugated ACC1 Monoclonal antibody (CL555-67373)

    IF Staining of HeLa using CL555-67373

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®555 ACC1 antibody (CL555-67373, Clone: 1A11G10 ) at dilution of 1:200.

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  • KD/KO Validated

CoraLite®555-conjugated ACC1 Monoclonal antibody

ACC1 Monoclonal Antibody for IF/ICC
Cat No. CL555-67373
Clone No.1A11G10

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Synonyms

ACAC, ACACA, ACC, ACC1, ACCA, Acetyl CoA carboxylase 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®555
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-67373 targets ACC1 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ACC1 fusion protein Ag17503 相同性解析による交差性が予測される生物種
Full Name acetyl-Coenzyme A carboxylase alpha
Calculated molecular weight 2383 aa, 275 kDa
Observed molecular weight 260-270 kDa
GenBank accession numberBC137287
Gene Symbol Acetyl-CoA Carboxylase 1
Gene ID (NCBI) 31
RRIDAB_3084629
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13085
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.

Protocols

Product Specific Protocols
IF protocol for CL555 ACC1 antibody CL555-67373Download protocol
Standard Protocols
Click here to view our Standard Protocols