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CoraLite® Plus 647-conjugated ACC1 Monoclonal antibody
ACC1 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
1A11G10
Cat no : CL647-67373
Synonyms
Validation Data Gallery
Tested Applications
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL647-67373 targets ACC1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | ACC1 fusion protein Ag17503 相同性解析による交差性が予測される生物種 |
Full Name | acetyl-Coenzyme A carboxylase alpha |
Calculated molecular weight | 2383 aa, 275 kDa |
Observed molecular weight | 260-270 kDa |
GenBank accession number | BC137287 |
Gene symbol | Acetyl-CoA Carboxylase 1 |
Gene ID (NCBI) | 31 |
RRID | AB_2920292 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 647 ACC1 antibody CL647-67373 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |