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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated ADRM1 Monoclonal ant (CL488-66464)

    IF Staining of HeLa using CL488-66464

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 ADRM1 antibody (CL488-66464, Clone: 1A1A3 ) at dilution of 1:200.

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CoraLite® Plus 488-conjugated ADRM1 Monoclonal antibody

ADRM1 Monoclonal Antibody for IF/ICC
Cat No. CL488-66464
Clone No.1A1A3

Host / Isotype

Mouse / IgG3

Reactivity

human

Applications

IF/ICC

Synonyms

adhesion regulating molecule 1, ADRM1, ARM 1, ARM1, GP110, hRpn13, Rpn13, Rpn13 homolog

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66464 targets ADRM1 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen ADRM1 fusion protein Ag24699 相同性解析による交差性が予測される生物種
Full Name adhesion regulating molecule 1
Calculated molecular weight 407 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC017245
Gene Symbol ADRM1
Gene ID (NCBI) 11047
RRIDAB_2934463
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16186
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The 26S proteasome is a key component of the ubiquitin-proteasome system, a process responsible for the majority of cellular protein degradation. hRpn13 (also termed ADRM1 or GP110) is a novel 46-kDa subunit of its 19S regulatory complex. hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity. Overexpression of hRpn13 promotes the activity of the ubiquitin-proteasome system and modulates the influence of osteoblasts on osteoclasts by controlling the stability of regulatory proteins in osteoblasts.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ADRM1 antibody CL488-66464Download protocol
Standard Protocols
Click here to view our Standard Protocols