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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated ADRM1 Monoclonal antibody (CL594-66464)

    IF Staining of HeLa using CL594-66464

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 ADRM1 antibody (CL594-66464, Clone: 1A1A3 ) at dilution of 1:200.

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CoraLite®594-conjugated ADRM1 Monoclonal antibody

ADRM1 Monoclonal Antibody for IF/ICC
Cat No. CL594-66464
Clone No.1A1A3

Host / Isotype

Mouse / IgG3

Reactivity

human

Applications

IF/ICC

Synonyms

adhesion regulating molecule 1, ADRM1, ARM 1, ARM1, GP110, hRpn13, Rpn13, Rpn13 homolog

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66464 targets ADRM1 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen ADRM1 fusion protein Ag24699 相同性解析による交差性が予測される生物種
Full Name adhesion regulating molecule 1
Calculated molecular weight 407 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC017245
Gene Symbol ADRM1
Gene ID (NCBI) 11047
RRIDAB_2934726
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16186
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The 26S proteasome is a key component of the ubiquitin-proteasome system, a process responsible for the majority of cellular protein degradation. hRpn13 (also termed ADRM1 or GP110) is a novel 46-kDa subunit of its 19S regulatory complex. hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity. Overexpression of hRpn13 promotes the activity of the ubiquitin-proteasome system and modulates the influence of osteoblasts on osteoclasts by controlling the stability of regulatory proteins in osteoblasts.

Protocols

Product Specific Protocols
IF protocol for CL594 ADRM1 antibody CL594-66464Download protocol
Standard Protocols
Click here to view our Standard Protocols