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  • Featured Product
  • KD/KO Validated

Apolipoprotein AI Polyclonal antibody

Apolipoprotein AI Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 14427-1-AP

Synonyms

APOA1, apolipoprotein A I, ApoA-I, Apo-AI, ApoAI

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human plasma using Apolipoprotein AI Polyclonal antibody (14427-1-AP)

    WB analysis of human plasma using 14427-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 14427-1-AP (Apolipoprotein AI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human ileum tissue using Apolipoprotein AI Polyclonal antibody (14427-1-AP)

    WB analysis of human ileum using 14427-1-AP

    human ileum tissue were subjected to SDS PAGE followed by western blot with 14427-1-AP (Apolipoprotein AI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using Apolipoprotein AI Polyclonal antibody (14427-1-AP)

    WB analysis of human brain using 14427-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14427-1-AP (Apolipoprotein AI antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Apolipoprotein AI Polyclonal antibody (14427-1-AP)

    IHC staining of human liver cancer using 14427-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14427-1-AP (Apolipoprotein AI antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Apolipoprotein AI Polyclonal antibody (14427-1-AP)

    IHC staining of human liver cancer using 14427-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14427-1-AP (Apolipoprotein AI antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using Apolipoprotein AI Polyclonal antibody (14427-1-AP)

    IHC staining of human liver using 14427-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14427-1-AP (Apolipoprotein AI antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Apolipoprotein AI Polyclonal antibody (14427-1-AP)

    IHC staining of human liver using 14427-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14427-1-AP (Apolipoprotein AI antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using Apolipoprotein AI Polyclonal antibody (14427-1-AP)

    IHC staining of human lung using 14427-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 14427-1-AP (Apolipoprotein AI antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using Apolipoprotein AI Polyclonal antibody (14427-1-AP)

    IHC staining of human lung using 14427-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 14427-1-AP (Apolipoprotein AI antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using Apolipoprotein AI Polyclonal antibody (14427-1-AP)

    IF Staining of mouse liver using 14427-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Apolipoprotein AI antibody (14427-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using Apolipoprotein AI Polyclonal antibody (14427-1-AP)

    IF Staining of mouse liver using 14427-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Apolipoprotein AI antibody (14427-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Apolipoprotein AI Polyclonal antibody (14427-1-AP)

    IF Staining of HeLa using 14427-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Apolipoprotein AI antibody (14427-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using Apolipoprotein AI Polyclonal antibody (14427-1-AP)

    FC experiment of HepG2 using 14427-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Apolipoprotein AI (14427-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inhuman blood tissue, human brain tissue, human plasma, human ileum tissue
Positive IHC detected inhuman liver cancer tissue, human liver tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14427-1-AP targets Apolipoprotein AI in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Apolipoprotein AI fusion protein Ag5793 相同性解析による交差性が予測される生物種
Full Name apolipoprotein A-I
Calculated molecular weight 31 kDa
Observed molecular weight 26-30 kDa
GenBank accession numberBC005380
Gene symbol APOA1
Gene ID (NCBI) 335
RRIDAB_2056524
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.

Protocols

Product Specific Protocols
WB protocol for Apolipoprotein AI antibody 14427-1-APDownload protocol
IHC protocol for Apolipoprotein AI antibody 14427-1-APDownload protocol
IF protocol for Apolipoprotein AI antibody 14427-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IP

Science

Enterically derived high-density lipoprotein restrains liver injury through the portal vein.

Authors - Yong-Hyun Han
View Article
mouseWB,IP

Neuron

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

Authors - Xiaohui Li
View Article
mouseWB

J Control Release

Lipid-mediated protein corona regulation with increased apolipoprotein A-I recruitment for glioma targeting

Authors - Yiwei Zhang
View Article
humanIHC

Br J Cancer

A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline.

Authors - Xiaohui Liu
View Article
humanWB

Theranostics

Exosome-mediated delivery of inflammation-responsive Il-10 mRNA for controlled atherosclerosis treatment

Authors - Te Bu
View Article

Br J Pharmacol

A novel apoA-I mimetic peptide suppresses atherosclerosis by promoting physiological HDL function in apoE-/- mice.

Authors - Sanhu Gou
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Alexandre (Verified Customer) (02-28-2019)

Detection of both human and mouse APOA1 protein by westernblotImmunoprecipitation of both human and mouse APOA1

  • Applications: Western Blot, Immunoprecipitation,
  • Primary Antibody Dilution: WB 1/1000
  • Cell Tissue Type: HepG2 / Mouse Liver
Apolipoprotein AI Antibody Western Blot,Immunoprecipitation, validation (WB 1/1000 dilution) in HepG2 / Mouse Liver (Cat no:14427-1-AP)