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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated Apolipoprotein AI Polyclonal antibody

Apolipoprotein AI Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-14427

Synonyms

APOA1, apolipoprotein A I, ApoA-I, Apo-AI, ApoAI

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 647-conjugated Apolipoprotein AI Po (CL647-14427)

    IF Staining of HepG2 using CL647-14427

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 647 Apolipoprotein AI antibody (CL647-14427) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 647-conjugated Apolipoprotein AI Po (CL647-14427)

    FC experiment of HepG2 using CL647-14427

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Apolipoprotein AI (CL647-14427) (red), or 0.4 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-14427 targets Apolipoprotein AI in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Apolipoprotein AI fusion protein Ag5793 相同性解析による交差性が予測される生物種
Full Name apolipoprotein A-I
Calculated molecular weight 31 kDa
Observed molecular weight 26-30 kDa
GenBank accession numberBC005380
Gene symbol APOA1
Gene ID (NCBI) 335
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 Apolipoprotein AI antibody CL647-14427Download protocol
FC protocol for CL Plus 647 Apolipoprotein AI antibody CL647-14427Download protocol
Standard Protocols
Click here to view our Standard Protocols