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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Apolipoprotein AI Polyclonal antibody

Apolipoprotein AI Polyclonal Antibody for IF/ICC, IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14427

Synonyms

APOA1, apolipoprotein A I, ApoA-I, Apo-AI, ApoAI

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using CoraLite® Plus 488-conjugated Apolipoprotein AI Po (CL488-14427)

    IF Staining of mouse liver using CL488-14427

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite® Plus 488 Apolipoprotein AI antibody (CL488-14427) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated Apolipoprotein AI Po (CL488-14427)

    IF Staining of HeLa using CL488-14427

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Apolipoprotein AI antibody (CL488-14427) at dilution of 1:100.

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Tested Applications

Positive IF-P detected inmouse liver tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-14427 targets Apolipoprotein AI in IF/ICC, IF-P applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Apolipoprotein AI fusion protein Ag5793 相同性解析による交差性が予測される生物種
Full Name apolipoprotein A-I
Calculated molecular weight 31 kDa
Observed molecular weight 26-30 kDa
GenBank accession numberBC005380
Gene symbol APOA1
Gene ID (NCBI) 335
RRIDAB_2919091
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Apolipoprotein AI antibody CL488-14427Download protocol
Standard Protocols
Click here to view our Standard Protocols