ARL13B Monoclonal antibody, PBS Only

HepG2 cells were subjected to SDS PAGE followed by western blot with 66739-1-Ig (ARL13B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66739-1-PBS in a different storage buffer formulation.

HEK-293 cells were subjected to SDS PAGE followed by western blot with 66739-1-Ig (ARL13B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66739-1-PBS in a different storage buffer formulation.

NCCIT cell were subjected to SDS PAGE followed by western blot with 66739-1-Ig (ARL13B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66739-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 66739-1-Ig (ARL13B antibody) at dilution of 1:60 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66739-1-PBS in a different storage buffer formulation.

Frozen tissue section of embryonic mouse heart (E12.5) was stained for cardiac troponin-T/cTnT (magenta, Cat. No 15513-1-AP), CD31/PECAM-1 (white), and Arl13B (yellow, Cat. No 66739-1-Ig) with DAPI as a counterstain for visualizing the nucleus (cyan). cTnT stains cardiomyocytes and was visualized with an Alexa Fluor 555 secondary antibody and psedocolored to magenta.CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody and psedocolored to white. Arl13B stains primary cilia and was visualized with an Alexa Fluor 488 secondary antibody and psedocolored to yellow. The Image and figure legends are intellectual property of @LAF_in_the_LAB. This data was developed using the same antibody clone with 66739-1-PBS in a different storage buffer formulation.

rozen tissue section of embryonic mouse heart (E12.5) was stained for cardiac troponin-T /cTnT (red, Cat. No 15513-1-AP), CD31/PECAM-1 (magenta), and Arl13B (green, Cat. No 66739-1-Ig) with DAPI as a counterstain for visualizing the nucleus (blue). cTnT stains cardiomyocytes and was visualized with an Alexa Fluor 555 secondary antibody. CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody. Arl13B stains primary cilia and was visualized with an Alexa Fluor 488 secondary antibody. The Image and figure legends are intellectual property of @LAF_in_the_LAB. This data was developed using the same antibody clone with 66739-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using ARL13B antibody (66739-1-Ig, Clone: 1H6C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ARL13B antibody (17711-1-AP, red). This data was developed using the same antibody clone with 66739-1-PBS in a different storage buffer formulation.