Validation Data Gallery

Filter: AllWBIF-FroIF/ICC

WB analysis of HepG2 using 66739-1-Ig
HepG2 cells were subjected to SDS PAGE followed by western blot with 66739-1-Ig (ARL13B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 66739-1-Ig
HEK-293 cells were subjected to SDS PAGE followed by western blot with 66739-1-Ig (ARL13B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of NCCIT using 66739-1-Ig
NCCIT cell were subjected to SDS PAGE followed by western blot with 66739-1-Ig (ARL13B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IF Staining of mouse heart using 66739-1-Ig
rozen tissue section of embryonic mouse heart (E12.5) was stained for cardiac troponin-T /cTnT (red, Cat. No 15513-1-AP), CD31/PECAM-1 (magenta), and Arl13B (green, Cat. No 66739-1-Ig) with DAPI as a counterstain for visualizing the nucleus (blue). cTnT stains cardiomyocytes and was visualized with an Alexa Fluor 555 secondary antibody. CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody. Arl13B stains primary cilia and was visualized with an Alexa Fluor 488 secondary antibody. The Image and figure legends are intellectual property of @LAF_in_the_LAB.
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rozen tissue section of embryonic mouse heart (E12.5) was stained for cardiac troponin-T /cTnT (red, Cat. No 15513-1-AP), CD31/PECAM-1 (magenta), and Arl13B (green, Cat. No 66739-1-Ig) with DAPI as a counterstain for visualizing the nucleus (blue). cTnT stains cardiomyocytes and was visualized with an Alexa Fluor 555 secondary antibody. CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody. Arl13B stains primary cilia and was visualized with an Alexa Fluor 488 secondary antibody. The Image and figure legends are intellectual property of @LAF_in_the_LAB.
IF Staining of mouse heart using 66739-1-Ig
Frozen tissue section of embryonic mouse heart (E12.5) was stained for cardiac troponin-T/cTnT (magenta, Cat. No 15513-1-AP), CD31/PECAM-1 (white), and Arl13B (yellow, Cat. No 66739-1-Ig) with DAPI as a counterstain for visualizing the nucleus (cyan). cTnT stains cardiomyocytes and was visualized with an Alexa Fluor 555 secondary antibody and psedocolored to magenta.CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody and psedocolored to white. Arl13B stains primary cilia and was visualized with an Alexa Fluor 488 secondary antibody and psedocolored to yellow. The Image and figure legends are intellectual property of @LAF_in_the_LAB.
×
Frozen tissue section of embryonic mouse heart (E12.5) was stained for cardiac troponin-T/cTnT (magenta, Cat. No 15513-1-AP), CD31/PECAM-1 (white), and Arl13B (yellow, Cat. No 66739-1-Ig) with DAPI as a counterstain for visualizing the nucleus (cyan). cTnT stains cardiomyocytes and was visualized with an Alexa Fluor 555 secondary antibody and psedocolored to magenta.CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody and psedocolored to white. Arl13B stains primary cilia and was visualized with an Alexa Fluor 488 secondary antibody and psedocolored to yellow. The Image and figure legends are intellectual property of @LAF_in_the_LAB.
IF Staining of hTERT-RPE1 using 66739-1-Ig
Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using ARL13B antibody (66739-1-Ig, Clone: 1H6C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ARL13B antibody (17711-1-AP, red).

ARL13B Monoclonal antibody

ARL13B Monoclonal Antibody for WB, IF/ICC, IF-Fro, ELISA

Cat No. 66739-1-Ig

Clone No.1H6C3

Publications(23)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig, canine and More (1)

Applications

WB, IHC, IF/ICC, IF-Fro, ELISA

Synonyms

ARL2-like protein 1, ARL2L1, ARL2 like protein 1, ADP-ribosylation factor-like protein 2-like 1, ADP-ribosylation factor-like protein 13B

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (23)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, HEK-293 cells, NCCIT cell
Positive IF-Fro detected in	mouse heart tissue
Positive IF/ICC detected in	hTERT-RPE1 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunofluorescence (IF)-FRO	IF-FRO : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IHC	See 1 publications below
IF	See 21 publications below

Product Information

66739-1-Ig targets ARL13B in WB, IHC, IF/ICC, IF-Fro, ELISA applications and shows reactivity with human, mouse, pig, canine samples.

Tested Reactivity	human, mouse, pig, canine
Cited Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	ARL13B fusion protein Ag12031 相同性解析による交差性が予測される生物種
Full Name	ADP-ribosylation factor-like 13B
Calculated molecular weight	48 kDa
Observed molecular weight	60 kDa
GenBank accession number	BC094725
Gene Symbol	ARL13B
Gene ID (NCBI)	200894
RRID	AB_2882088
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q3SXY8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).

Protocols

Product Specific Protocols
WB protocol for ARL13B antibody 66739-1-Ig	Download protocol
IF protocol for ARL13B antibody 66739-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF,WB	Nat Commun INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex Authors - Lauren G. Mascibroda View Article
human	WB	Elife Multiple ciliary localization signals control INPP5E ciliary targeting Authors - Dario Cilleros-Rodriguez View Article
human	IF	Sci Rep Targeted exon skipping rescues ciliary protein composition defects in Joubert syndrome patient fibroblasts. Authors - Elisa Molinari View Article
rat	IF	eNeuro CCP1, a Tubulin Deglutamylase, Increases Survival of Rodent Spinal Cord Neurons following Glutamate-Induced Excitotoxicity. Authors - Yasmin H Ramadan View Article
human	IF	PLoS One Embryonic and foetal expression patterns of the ciliopathy gene CEP164. Authors - L A Devlin View Article
mouse	IF	Life Sci Alliance HTR6 and SSTR3 ciliary targeting relies on both IC3 loops and C-terminal tails. Authors - Pablo Barbeito View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HepG2 using 66739-1-Ig

WB analysis of HEK-293 using 66739-1-Ig

WB analysis of NCCIT using 66739-1-Ig

IF Staining of mouse heart using 66739-1-Ig

IF Staining of mouse heart using 66739-1-Ig

IF Staining of hTERT-RPE1 using 66739-1-Ig

ARL13B Monoclonal antibody

ARL13B Monoclonal Antibody for WB, IF/ICC, IF-Fro, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex

Multiple ciliary localization signals control INPP5E ciliary targeting

Targeted exon skipping rescues ciliary protein composition defects in Joubert syndrome patient fibroblasts.

CCP1, a Tubulin Deglutamylase, Increases Survival of Rodent Spinal Cord Neurons following Glutamate-Induced Excitotoxicity.

Embryonic and foetal expression patterns of the ciliopathy gene CEP164.

HTR6 and SSTR3 ciliary targeting relies on both IC3 loops and C-terminal tails.