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ARL13B Monoclonal antibody

ARL13B Monoclonal Antibody for WB, IF/ICC, IF-Fro, ELISA
Cat No. 66739-1-Ig
Clone No.1H6C3

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig, canine and More (1)

Applications

WB, IHC, IF/ICC, IF-Fro, ELISA

ARL2-like protein 1, ARL2L1, ARL2 like protein 1, ADP-ribosylation factor-like protein 2-like 1, ADP-ribosylation factor-like protein 13B

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  150 μL, 2200 ug/ml
20 μL, 2200 ug/ml
150 μL, 2200 ug/ml

¥67,000/ 150 μL

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テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, NCCIT cell
Positive IF-Fro detected inmouse heart tissue
Positive IF/ICC detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)-FROIF-FRO : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66739-1-Ig targets ARL13B in WB, IHC, IF/ICC, IF-Fro, ELISA applications and shows reactivity with human, mouse, pig, canine samples.

Tested Reactivity human, mouse, pig, canine
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ARL13B fusion protein Ag12031 相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor-like 13B
Calculated molecular weight 48 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC094725
Gene Symbol ARL13B
Gene ID (NCBI) 200894
RRIDAB_2882088
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ3SXY8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).

Protocols

Product Specific Protocols
WB protocol for ARL13B antibody 66739-1-IgDownload protocol
IF protocol for ARL13B antibody 66739-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF,WB

Nat Commun

INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex

Authors - Lauren G. Mascibroda
humanWB

Elife

Multiple ciliary localization signals control INPP5E ciliary targeting

Authors - Dario Cilleros-Rodriguez
humanIF

Sci Rep

Targeted exon skipping rescues ciliary protein composition defects in Joubert syndrome patient fibroblasts.

Authors - Elisa Molinari
ratIF

eNeuro

CCP1, a Tubulin Deglutamylase, Increases Survival of Rodent Spinal Cord Neurons following Glutamate-Induced Excitotoxicity.

Authors - Yasmin H Ramadan
humanIF

PLoS One

Embryonic and foetal expression patterns of the ciliopathy gene CEP164.

Authors - L A Devlin
mouseIF

Life Sci Alliance

HTR6 and SSTR3 ciliary targeting relies on both IC3 loops and C-terminal tails.

Authors - Pablo Barbeito
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