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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated ARL13B Monoclonal antibody

ARL13B Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Canine

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H6C3

Cat no : CL488-66739

Synonyms

ARL13B, ARL2 like protein 1, ARL2L1, DKFZp686E2075, DKFZp686L2472, DKFZp686M2074, DKFZp761H079, JBTS8

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using CoraLite® Plus 488-conjugated ARL13B Monoclonal an (CL488-66739)

    IF Staining of hTERT-RPE1 using CL488-66739

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite® Plus 488 ARL13B antibody (CL488-66739, Clone: 1H6C3 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using CoraLite® Plus 488-conjugated ARL13B Monoclonal an (CL488-66739)

    IF Staining of MDCK using CL488-66739

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CL488-66739 (ARL13B antibody) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inhTERT-RPE1 cells, MDCK cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66739 targets ARL13B in IF/ICC applications and shows reactivity with Human, Canine samples.

Tested Reactivity Human, Canine
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ARL13B fusion protein Ag12031 相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor-like 13B
Calculated molecular weight 48 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC094725
Gene symbol ARL13B
Gene ID (NCBI) 200894
RRIDAB_2883377
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ARL13B antibody CL488-66739Download protocol
Standard Protocols
Click here to view our Standard Protocols