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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated ASNA1 Monoclonal antibody

ASNA1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B7C11

Cat no : CL488-66346

Synonyms

TRC40, ATPase GET3, ASNA 1, Arsenite-stimulated ATPase, Arsenical pump-driving ATPase

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated ASNA1 Monoclonal ant (CL488-66346)

    IF Staining of HeLa using CL488-66346

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 ASNA1 antibody (CL488-66346, Clone: 1B7C11 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66346 targets ASNA1 in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ASNA1 fusion protein Ag7936 相同性解析による交差性が予測される生物種
Full Name arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)
Calculated molecular weight 38.7 kDa
Observed molecular weight 37-41 kDa
GenBank accession numberBC002651
Gene symbol ASNA1
Gene ID (NCBI) 439
RRIDAB_2883314
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ASNA1 (also known as TRC40) is a highly conserved ATPase involved in efflux of arsenite and antimonite. Reduced ASNA1 expression is associated with significant inhibition of cell growth, increased apoptosis and increased sensitivity to arsenite.Thus ASNA1 is proposed to be a target to overcome resistance to cancer chemotherapy. In addition, ASNA1 has been identified as an ER targeting factor for tail-anchored proteins in the posttranslational membrane insertion pathway.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ASNA1 antibody CL488-66346Download protocol
Standard Protocols
Click here to view our Standard Protocols