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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CoraLite®594-conjugated ASNA1 Monoclonal antibody (CL594-66346)

    IF Staining of HEK-293 using CL594-66346

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite®594 ASNA1 antibody (CL594-66346, Clone: 1B7C11 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite®594-conjugated ASNA1 Monoclonal antibody (CL594-66346)

    FC experiment of HEK-293 using CL594-66346

    1X10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human ASNA1 (CL594-66346, Clone:1B7C11) (red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CoraLite®594-conjugated ASNA1 Monoclonal antibody

ASNA1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66346
Clone No.1B7C11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Synonyms

TRC40, ATPase GET3, ASNA 1, Arsenite-stimulated ATPase, Arsenical pump-driving ATPase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66346 targets ASNA1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ASNA1 fusion protein Ag7936 相同性解析による交差性が予測される生物種
Full Name arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)
Calculated molecular weight 38.7 kDa
Observed molecular weight 37-41 kDa
GenBank accession numberBC002651
Gene Symbol ASNA1
Gene ID (NCBI) 439
RRIDAB_2883564
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43681
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ASNA1 (also known as TRC40) is a highly conserved ATPase involved in efflux of arsenite and antimonite. Reduced ASNA1 expression is associated with significant inhibition of cell growth, increased apoptosis and increased sensitivity to arsenite.Thus ASNA1 is proposed to be a target to overcome resistance to cancer chemotherapy. In addition, ASNA1 has been identified as an ER targeting factor for tail-anchored proteins in the posttranslational membrane insertion pathway.

Protocols

Product Specific Protocols
IF protocol for CL594 ASNA1 antibody CL594-66346Download protocol
FC protocol for CL594 ASNA1 antibody CL594-66346Download protocol
Standard Protocols
Click here to view our Standard Protocols