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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated ATPB Monoclonal antibody

ATPB Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A11B9

Cat no : CL594-66600

Synonyms

ATPSB, ATPMB, ATP5F1B, ATP5B, ATP synthase F1 subunit beta

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated ATPB Monoclonal antibody (CL594-66600)

    IF Staining of HepG2 using CL594-66600

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 ATPB antibody (CL594-66600, Clone: 1A11B9 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated ATPB Monoclonal antibody (CL594-66600)

    FC experiment of HepG2 using CL594-66600

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human ATPB (CL594-66600, Clone:1A11B9) (red), or 0.8 ug CoraLite®594 Mouse IgG2b Isotype Control (MPC-11) (CL594-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

CL594-66600 targets ATPB in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ATPB fusion protein Ag11177 相同性解析による交差性が予測される生物種
Full Name ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
Calculated molecular weight 529 aa, 57 kDa
Observed molecular weight45-57 kDa
GenBank accession numberBC016512
Gene symbol ATPB
Gene ID (NCBI) 506
RRIDAB_2920015
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP5B, also named as ATPMB and ATPSB, belongs to the ATPase alpha/beta chains family. It is a high affinity HDL receptor for apolipoprotein A1. ATP5B is a subunit of mitochondrial ATP synthase (F1F0 ATP synthase or Complex V ). ATP5B has a calculated molecular mass of 57 kDa and ~5 kDa transit peptide can be removed in the mature form.

Protocols

Product Specific Protocols
IF protocol for CL594 ATPB antibody CL594-66600Download protocol
FC protocol for CL594 ATPB antibody CL594-66600Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Exp Ther Med

Targeting translocator protein protects against myocardial ischemia/reperfusion injury by alleviating mitochondrial dysfunction

Authors - Chenghao Wen
View Article