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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated Albumin Monoclonal a (CL488-66051)

    IF Staining of HepG2 using CL488-66051

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488-conjugated Albumin antibody (CL488-66051, Clone: 4A1C11 ) at dilution of 1:400.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated Albumin Monoclonal a (CL488-66051)

    FC experiment of HepG2 using CL488-66051

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Albumin (CL488-66051, Clone:4A1C11) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 488-conjugated Albumin Monoclonal antibody

Albumin Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66051
Clone No.4A1C11
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig and More (1)

Applications

IF/ICC, FC (Intra)

Synonyms

ALB, Serum albumin, HSA, 4A1C11

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

CL488-66051 targets Albumin in IF/ICC, FC (Intra) applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Albumin fusion protein Ag9885 相同性解析による交差性が予測される生物種
Full Name albumin
Calculated molecular weight 609 aa, 69 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC034023
Gene Symbol Albumin
Gene ID (NCBI) 213
RRIDAB_2883241
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02768
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Albumin antibody CL488-66051Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Mol Cell

WWC proteins mediate LATS1/2 activation by Hippo kinases and imply a tumor suppression strategy.

Authors - Sixian Qi
View Article