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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated Albumin Monoclonal antibody

Albumin Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC, IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4A1C11

Cat no : CL594-66051

Synonyms

ALB, Serum albumin, HSA, 4A1C11

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using CoraLite®594-conjugated Albumin Monoclonal antibod (CL594-66051)

    IF Staining of mouse liver using CL594-66051

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite®594 Albumin antibody (CL594-66051, Clone: 4A1C11 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated Albumin Monoclonal antibod (CL594-66051)

    IF Staining of HepG2 using CL594-66051

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 Albumin antibody (CL594-66051, Clone: 4A1C11 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated Albumin Monoclonal antibod (CL594-66051)

    FC experiment of HepG2 using CL594-66051

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Albumin (CL594-66051, Clone:4A1C11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inmouse liver tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66051 targets Albumin in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Albumin fusion protein Ag9885 相同性解析による交差性が予測される生物種
Full Name albumin
Calculated molecular weight 609 aa, 69 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC034023
Gene symbol Albumin
Gene ID (NCBI) 213
RRIDAB_2883493
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071)

Protocols

Product Specific Protocols
IF protocol for CL594 Albumin antibody CL594-66051Download protocol
FC protocol for CL594 Albumin antibody CL594-66051Download protocol
Standard Protocols
Click here to view our Standard Protocols