CoraLite® Plus 488-conjugated Alpha Actinin Monoclonal antibody
Alpha Actinin Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG2b
Reactivity
Human, rat, mouse
Applications
WB
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1E2E1
Cat no : CL488-66895
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, HeLa cells, RAW 264.7 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:8000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-66895 targets Alpha Actinin in WB applications and shows reactivity with Human, rat, mouse samples.
Tested Reactivity | Human, rat, mouse |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | Alpha Actinin fusion protein Ag28383 相同性解析による交差性が予測される生物種 |
Full Name | actinin, alpha 1 |
Calculated molecular weight | 103 kDa |
Observed molecular weight | 100-105 kDa |
GenBank accession number | BC015766 |
Gene symbol | Alpha Actinin |
Gene ID (NCBI) | 87 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.
Protocols
Product Specific Protocols | |
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WB protocol for CL Plus 488 Alpha Actinin antibody CL488-66895 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |