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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated Alpha Actinin Monoclonal antibody

Alpha Actinin Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E2E1

Cat no : CL594-66895

Synonyms

actinin, alpha 1, ACTN1, Alpha Actinin, Alpha actinin 1, F actin cross linking protein, FLJ40884, FLJ54432, Non muscle alpha actinin 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using CoraLite®594-conjugated Alpha Actinin Monoclonal a (CL594-66895)

    IF Staining of C2C12 using CL594-66895

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using CoraLite®594 Alpha Actinin antibody (CL594-66895, Clone: 1E2E1 ) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inC2C12 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66895 targets Alpha Actinin in IF/ICC applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Alpha Actinin fusion protein Ag28383 相同性解析による交差性が予測される生物種
Full Name actinin, alpha 1
Calculated molecular weight 103 kDa
Observed molecular weight 100-105 kDa
GenBank accession numberBC015766
Gene symbol Alpha Actinin
Gene ID (NCBI) 87
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.

Protocols

Product Specific Protocols
IF protocol for CL594 Alpha Actinin antibody CL594-66895Download protocol
Standard Protocols
Click here to view our Standard Protocols