• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated p130Cas / BCAR1 Monoclonal antibody

p130Cas / BCAR1 Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D11C10

Cat no : CL594-67215

Synonyms

BCAR1, 2D11C10, CASS1, CAS1, CAS

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CoraLite®594-conjugated p130Cas / BCAR1 Monoclonal (CL594-67215)

    IF Staining of human breast cancer using CL594-67215

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 BCAR1 antibody (CL594-67215, Clone: 2D11C10 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of A431 cells using CoraLite®594-conjugated p130Cas / BCAR1 Monoclonal (CL594-67215)

    FC experiment of A431 using CL594-67215

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human p130Cas / BCAR1 (CL594-67215, Clone:2D11C10) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive IF-P detected inhuman breast cancer tissue
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67215 targets p130Cas / BCAR1 in IF-P, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen p130Cas / BCAR1 fusion protein Ag10604 相同性解析による交差性が予測される生物種
Full Name breast cancer anti-estrogen resistance 1
Calculated molecular weight 870 aa, 93 kDa
GenBank accession numberBC062556
Gene symbol BCAR1
Gene ID (NCBI) 9564
RRIDAB_2920097
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL594 p130Cas / BCAR1 antibody CL594-67215Download protocol
FC protocol for CL594 p130Cas / BCAR1 antibody CL594-67215Download protocol
Standard Protocols
Click here to view our Standard Protocols