Validation Data Gallery
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Tested Applications
| Positive FC (Intra) detected in | A431 cells |
| Positive FC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL647-67215 targets p130Cas / BCAR1 in FC (Intra) applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | p130Cas / BCAR1 fusion protein Ag10604 相同性解析による交差性が予測される生物種 |
| Full Name | breast cancer anti-estrogen resistance 1 |
| Calculated molecular weight | 870 aa, 93 kDa |
| Observed molecular weight | 116 kDa |
| GenBank accession number | BC062556 |
| Gene Symbol | BCAR1 |
| Gene ID (NCBI) | 9564 |
| RRID | AB_2935074 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P56945 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 p130Cas / BCAR1 antibody CL647-67215 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |