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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated C1qC Monoclonal antibody

C1qC Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C5B4

Cat no : CL488-66268

Synonyms

C1Q C, C1QC, C1QG, FLJ27103

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using CoraLite® Plus 488-conjugated C1qC Monoclonal anti (CL488-66268)

    IF Staining of HUVEC using CL488-66268

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using CoraLite® Plus 488 C1qC antibody (CL488-66268, Clone: 1C5B4 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66268 targets C1qC in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen C1qC fusion protein Ag10233 相同性解析による交差性が予測される生物種
Full Name complement component 1, q subcomponent, C chain
Calculated molecular weight 245 aa, 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC009016
Gene symbol C1QC
Gene ID (NCBI) 714
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodThiophilic affinity chromatograph
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qC which is the C-chain polypeptide of human complement subcomponent C1q.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 C1qC antibody CL488-66268Download protocol
Standard Protocols
Click here to view our Standard Protocols