Validation Data Gallery
Tested Applications
| Positive IF-P detected in | mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-66211 targets CD13 in IF-P applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD13 fusion protein Ag5976 相同性解析による交差性が予測される生物種 |
| Full Name | alanyl (membrane) aminopeptidase |
| Calculated molecular weight | 110 kDa |
| GenBank accession number | BC058928 |
| Gene Symbol | CD13 |
| Gene ID (NCBI) | 290 |
| ENSEMBL Gene ID | ENSG00000166825 |
| RRID | AB_2883537 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P15144 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD13, also named as APN, ANPEP(aminopeptidase N) or PEPN, is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174).And this antibody is conjugated with CL594(Ex/Em 593 nm/614 nm).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 CD13 antibody CL594-66211 | Download protocol |
| FC protocol for CL594 CD13 antibody CL594-66211 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |