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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Atlantic Blue™ Anti-Human CD18 (TS1/18)

CD18 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

Atlantic Blue™ Fluorescent Dye

CloneNo.

TS1/18

Cat no : AB-65190

Synonyms

CD18, Integrin beta 2, ITGB2, LAD, LCAMB, LFA 1, MAC 1, MF17, MFI7

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Atlantic Blue™ Anti-Human CD18 (TS1/18) (AB-65190)

    FC experiment of human PBMCs using AB-65190

    1X10^6 human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human CD18 (AB-65190, Clone:TS1/18) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Atlantic Blue™ Anti-Human CD18 (TS1/18) (AB-65190)

    FC experiment of human PBMCs using AB-65190

    1X10^6 human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human CD18 (AB-65190, Clone:TS1/18) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

AB-65190 targets CD18 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human Beta 2 Integrin 相同性解析による交差性が予測される生物種
Full Name integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
Calculated molecular weight 85 kDa
GenBank accession numberBC005861
Gene symbol CD18
Gene ID (NCBI) 3689
Conjugate Atlantic Blue™ Fluorescent Dye
Excitation/Emission maxima wavelengths404 nm / 458 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).

Protocols

Product Specific Protocols
FC protocol for Atlantic Blue™ CD18 antibody AB-65190Download protocol
Standard Protocols
Click here to view our Standard Protocols