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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488 Anti-Human CD18 (TS1/18)

CD18 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

TS1/18

Cat no : CL488-65190

Synonyms

CD18, Integrin beta 2, ITGB2, LAD, LCAMB, LFA 1, MAC 1, MF17, MFI7

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using CoraLite® Plus 488 Anti-Human CD18 (TS1/18) (CL488-65190)

    FC experiment of human peripheral blood lymphocytes using CL488-65190

    1X10^6 human peripheral blood lymphocytes were surface stained with 5.00 ul/test CoraLite® Plus 488-conjugated Anti-Human CD18 (CL488-65190, Clone: TS1/18) (red) or isotype control antibody (blue). Cells were not fixed.

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Tested Applications

Positive FC detected inhuman peripheral blood lymphocytes

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65190 targets CD18 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human Beta 2 Integrin 相同性解析による交差性が予測される生物種
Full Name integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
Calculated molecular weight 85 kDa
GenBank accession numberBC005861
Gene symbol CD18
Gene ID (NCBI) 3689
RRIDAB_2883213
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CD18 antibody CL488-65190Download protocol
Standard Protocols
Click here to view our Standard Protocols